Application

Anti-SLFN11 Antibody, clone 4G9 is an antibody against SLFN11 for use in western blotting & IP.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Western Blotting/Immunoprecipitation Analysis: A representative lot immunoprecipitated SLFN11 from transfected HEK293 cell lysates. The same lot was used to detect SLFN11 in Western Blotting (Prof. Michael David, University of California, San Diego.).

Electron Mobility Shift Assay Analysis: A representative lot from an independent laboratory detected SLFN11 bound t-RNA (Li, M., et al. (2012). Nature. 491(7422):125-128.).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SLFN11, also known as Schlafen family member 11, and encoded by the gene SLFN11, is an interesting protein that is part of our innate defense against retroviruses. The protein is induced by interferon and acts to inhibit the translation of retrovirus specific proteins. SLFN11 also plays a role in cell cycle arrest and apoptosis induction, particularly in response to DNA damage. SLFN11 is localized to the nucleus and is widely expressed and up regulated in various cancers including ovarian and adenocarcinomas, and recent work suggests that SLFN11 may behave as a biomarker for prediction of response to DNA-damaging agents in the clinical setting.

Immunogen

Recombinant protein corresponding to human SLFN11.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293T cell lysates transfected with SLFN11 or a negative control.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SFN11 in HEK293T cell lysates transfected with SLFN11 and not in HEK293T cell lysates transfected with a negative control.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~100 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.